Services and Instruments

    Seahorse XFe-96 Extracellular Flux Analyzer

    Website: Seahorse Bioscience

    A fast, comprehensive method for quantitatively assessing respiration and glycolysis in living cell cultures

    Cellular respiration and glycolysis affect the immediate extracellular environment, yielding measurable changes in O2 concentration and pH. Seahorse Bioscience's microplate-based ECF analyzer makes real-time measurements (every ~5 seconds) of oxygen consumption and extracellular acidification using an array of solid-state sensor probes.  By isolating and sampling a minute volume of medium above a monolayer of cells, the instrument makes and displays highly sensitive, rapid measurements.

  • Perform time-resolved measurements of a single population of cells over a period of minutes, hours, or, in some cases, days
  • Simultaneously measure oxygen consumption rate (OCR, nM/min), and extracellular acidification rate (ECAR, mpH/min)
  • Because the measurement methods are passive, cells can be reused, retested, and remeasured
  • Specially-designed microplates allow for customization of experimental conditions and injection of up to four different compounds into the medium at user-specified intervals (e.g., antibiotics, toxins, nutrients)
  • Intuitive, user-friendly control software makes planning and executing an experiment very straightforward
  • Powerful analytical tools built into instrument software, so that the user can observe, perform calculations on, and sift through the data as it's collected
  • Very little extra prep work: seed cells onto the XF microplate and incubate as usual; when it's time for the experiment, simply switch to unbuffered medium, incubate without CO2 for ~an hour via the XF Prep Station, set up any custom conditions and treatments, and start the run
  • Automated Prep Station consistently and precisely performs all the little steps that add hours and frustration to experiments, acting as a medium changer, cell washer, and non-CO2 incubator
  • Average total runtime (excluding cell prep): 3-4 hours
  • Several different stress test kits available for common experimental conditions, containing precalibrated, pretested reagents