- Detection range: 250-850nm
- Instrument is self-calibrating and completely automated, aside from sample loading
- Scans quickly and with extremely high sensitivity; signal-to-noise of at least 5000:1
- Double-grating emission monochromator isolates the relevant signal in highly-scattering samples
- Peltier controller available for precise temperature control during anisotropy and phase-transition experiments
- Measure intramolecular distances via Foerster resonance energy transfer (FRET)
- Customize all aspects of your experiment using HORIBA's FluorEssence software package, with real-time monitoring and modification of parameters, automated protocols, and powerful analysis tools
Services and Instruments
HORIBA FluoroLog-3 Spectrofluorometer
Website: HORIBA Jobin Yvon, Inc.
Perform all manner of fluorescence-based experiments in wavelengths from middle-UV to near-IR
Fluorescence spectroscopy is a powerful analytical tool for understanding and monitoring molecule conformation and interaction. Because different functional groups and bond types will exhibit different fluorescence signatures, this technique can help you to understand the structure and chemistry of your molecule of interest.
Spectrofluorometry can also provide information on conformational changes due to changes in temperature and pH, chemical denaturation, and ligand interaction, and fluorescence polarization techniques can be used to monitor binding and association.