Services and Instruments

    Jasco J-1500 Circular Dichroism Spectrometer

    Website: Jasco Analytical Instruments, Inc.

    Characterize the structure and conformation of biomolecules

    Chiroptical stereochemistry is used for structural analysis of biomolecules.  The instrument produces circularly polarized light, which passes through molecules in solution; the chirality of their components and of the molecules themselves causes minute differences in both velocity and wavelength for right- versus left-circularly polarized light.  This difference is measured and reported, and can yield crucial data regarding the structure and conformation of a molecule.  Any functional group that absorbs light will have a distinct CD signature within each absorption band, making CD spectroscopy broadly useful in investigating virtually any biomolecule, from small organic molecules and nucleic acids to higher-weight proteins, polymers, and polypeptides.

    Moreover, the secondary and tertiary structural components of the molecule impart a CD signature in the UV spectrum.  Though this technique does not provide a piece-by-piece "architectural plan" of the molecule, it does provide an indication of the percentage of the molecule that is in each conformation (α-helix, β-sheet, β-turn, etc.), which can then be used in tandem with other techniques to deduce the complete structure.  Using these principles, denaturation and other conformational changes, along with the thermodynamics of such changes, may also be studied within this spectral range. 

    • Detection range: 163-950nm
    • Study conformational stability of a macromolecule under chemical and thermal stress
    • Determine whether molecular binding and other interactions alter the conformation of a protein
    • Negligible stray light (0.0003% at 200 nm) and noise (<0.01 mdeg at all wavelengths)
    • Rapid scan speed; can read across its full range in a few seconds
    • Attached Peltier unit for precise temperature control
    • See the full specifications here
    • Jasco's Spectra Manager suite integrates data acquisition, presentation, and low-level analysis, while the OLIS GlobalWorks software package is also available for detailed model-based analysis