- True in-solution method — no chemical tagging or immobilization of binding components
- Can be used with turbid or colored solutions and particulate suspensions
- Experiments are entirely computer-controlled, including injection parameters and mixing
- Fast and knowledge-rich compared to other analytical methods
- Directly measures heat change associated with binding
GE MicroCal iTC200
Website: GE Healthcare Life Sciences
Further information: iTC200 Primer
Study the thermodynamics of binding and other chemical interactions in solution, without labeling or immobilization
Isothermal Titration Calorimetry (ITC) is a thermodynamic technique used for the determination of binding constants, reaction stoichiometry, and thermodynamic profile of binding reactions, including enthalpy (DH) and entropy (DS), and has become the method of choice for characterizing biomolecular interactions.
When substances bind, heat is either generated or absorbed. Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy ( ΔH) and entropy ( ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment. In ITC, a syringe containing a ligand solution is titrated into a cell containing a solution of the macromolecule at constant temperature. When ligand is injected into the cell, the two materials interact, and heat is released or absorbed in direct proportion to the amount of binding. As the macromolecule in the cell becomes saturated with ligand, the heat signal diminishes until only background heat of dilution is observed.